Psilocybin and psilocin extraction from mushrooms

[G.Patton]

~20-30%

 

[Neo156773]

Do you know if 4-aco-dmt freebase is soluble in ethyl acetate and if yes how many mg/ml ?

and is ethyl acetate a nice solvent to dissolve a mix of psilocin freebase & acetic anhydride (1,1mol e.q) & sodium acetate (0,1 mol e.q) ( acetylation process of psilocin —> 4-aco-dmt)

maybe for better yield performing acetylation process under influence of nitrogen ballon to reduce oxidation and humidity ) -> then after acetylation neutralize tensing acetic anhydride with water to the ethyl acetate (which contains acetic anhydride & Psilocin freebase & sodium acetate Then adding a bit sodium hydroxide to pH 10 and then extracting 4aco dmt freebase again (should hopefully end in ethyl acetate layer/phase). Then drying ethyl acetate with sodium sulfate and then rotary evoporation to obtain 4aco dmt freebase.
Then dissolving in hot acetone (jar 1) und also dissolve the right amount of fumaric acid in acetone (jar 2). Then mixing (jar 1 and 2) and stirr for a few mins on a magnetic stirrer and then rotary evaporation to obtain 4-aco-dmt fumarate ( for long term storage free from humidity/light/oxygen & maybe in the refrigerator ) what do you think ?

 

[G.Patton]

Hi. Sorry for long reply. Yes, you can. Procedure is same.

 

[Neo156773]

It does not work for amanita
A/B-tek does not work because Muscimol freebase is still not soluble in organic solvents like ethyl acetate.
I tried it.
You can try to extract with 10% acetic acid and then defat with xylene/ethyl acetate.
But basing with ammonia (pH 8-10) and then extracting with organic solvent won’t work

 

[Osmosis Vanderwaal]

There are no Amanita species mushrooms that are psilocybian. Amanita muscaria is a deleriant and the active substituents are muscimol and ibotenic acid

 

[Neo156773]

Nobody said that amanitas contain psilocybin?
he/she asked if it’s possible to do an a/b tek for amanitas ( for Muscimole hydrochloride I guess) and the answer is no

 

[Neo156773]

Muscimole is very polar even as freebase. It will prefer the aqueous layer and not the organic layer (toluene/naphta/xylene/dcm/ethyl acetate).
That’s the problem.
I tried it with xylene and yield was very bad

 

[Rutherford]

great explanation! congrats, I'll use this when my shrooms grow up soon.
I have a question, is there any difference between the extraction with glacial acetic acid and the alcohol extraction? In my case is a bit hard to obtain glacial acetic acid. thanks in advance!

 

[autodidact6]

i have no idea what your talking about apart from alcohol extraction ....i am not a chemist ,i am just another hippy trying to figure out things.thanks in advance!

 

[Osmosis Vanderwaal]

Yes the extraction with GAA I assumevis turning it into 4-aco- dmt, aka 4-ho-dmt acetate. The alcohol extraction is leaving it an alcohol (which it is naturally).
If an average dose of psilocin/psilocybin is ...20mg, I don't see how you could get that on an lsd tab size piece of anything

 

[Osmosis Vanderwaal]

I'm not a chemist. It could instead make 4-ho-dimethyltryptamide. I see there are references to carboxolic acids creating N,n- dimethyltryptamide and the reaction of a carboxolic acid to an aminevis an amide

 

[Mr Gonzo]

I have a couple of questions regarding this these techs.

1st, the acid to base tech yields psilocin. My Q is the mushrooms produce both this and psilocybin in the fruit, if the extraction is done via A to B would this not contain the spectrum of tryptamines Inc beatosicin?

2nd, sticking with the A to B tech. Is the synth not more productive like using Shulkin's TIHKAL tech to produce the compound being ripped out of the mushrooms, granted it requires more skill/ability. Still yield's would be greater than the extraction, and the synth will only produce the selected compound!

3rd, this concerns the alcohol extraction. I am sure many of the people on here will be familiar the that bullshit "Crystals Of The Gods" alcohol extraction claiming to yield psilocin crystals!
My point is using the alcohol tincture in jello shots, now that is a banging way to turn that extraction into a usable product. When I first got into mycology & chemistry about the same time some time ago now, I was so annoyed in wasting my precious mushrooms is such a crap lying tech claiming to get a crystalline product out of.

Thanks for the guide though General! Clears all that stuff up clear and concise.

 

[Osmosis Vanderwaal]

I'm not a chemist. It could instead make 4-ho-dimethyltryptamide. I see there are references to carboxolic acids creating N,n- dimethyltryptamide and the reaction of a carboxolic acid to an aminevis an amide. . I wrote this yesterday and it didn't send
@Mr Gonzo, I assume an A/B would de'phosporlate psilocybin and nor baocystin, , if your stomach does then an A/B certainly would. Baocystin is not often times detected in psilocybin mushrooms. I've never heard of it in a cubensis for sure. P. Baocystis and azurescens yes, but these wood lovers on a phylogenetic tree aren't anywhere near cubensis. In the current sense of Psilocybe they are as far apart as they can be

 

[Mr Gonzo]

I'm a moron haha, and I am not a chemist either Frit Buchner. I am in the second year of a chemistry degree and failed this summers exam and have to sit the entire year again, still you obviously have taught yourself well. Swear these are things that will stop me passing uni with a decent mark.
Now looking at what I was saying the charge on the phosphate will be cleaved off during the extraction. I appreciate the mistake being made obvious as this is how we learn sometimes, rather this than a lab exploding on me!

As with most questions & answers, answers lead to more questions especially when the question was not correct to start with. How would the psilocybin be extracted from the bio mass, would this be a straight to base extraction?

 

[Osmosis Vanderwaal]

You know I've never even considered the idea of the mechanics of the extraction, but I see psilocin is very soluble in ethanol and barely soluble in water, so it is a base extraction, using alcohol and clearly would be a poor choice of an extraction solvent (for the base) then there's psilocybin the phosphate Ester, it is soluble in water, barely soluble in ethanol and stable and forms crystals according to several reliable accounts. I suppose the fumerate or other acid salt would be water soluble and form crystals also, which would be a "straight to acid " extraction. Makes you think. Clearly after you did 2 pulls with ethanol you would want to either neutralize the phosphate or do a water extraction. I always did a water extraction on the 3rd pull and when it hit the alcohol as I combined them, it make a murky unappetizing mud cocktail..
THEN THERE WAS THIS ONE TIME I came across a HUGE HAUL of big laughing gyms (Gymnopilus spectacles at the time, but there was recently a lot of genetic work done on the genus that I haven't read) which are active in the US and bruise green, but the European equivalent Gymnopilus junonius isn't active. Anyway, I was poor at the time, but Gymnopilus are notoriously bitter, so I dried them in a dehydrator and did a water extraction on 45 grams dry ( eq. To 1 pound fresh) as they are not reported to be very potent. I layer there on the couch feeling like I was being stabbed all over my body with hot pokers for the first hour before I did have a fair trip equivocal to 3-4 grams of cubensis. Trying times, those were. UNFORTUNATELY I have more negative stories than positive ones about psilocybin mushrooms. I abused them too much. I would take them on a Tuesday and sit around the house and drink beer and watch TV. I tripped mushrooms probably 75 times the first year I was successful at cultivating them. I suggest saving the mushrooms for special occasions a few times a year AT THE MOST.
Extraction is definitely the way to go. Comes on in 20 minutes instead of waiting an hour. Mushrooms are high in chitosan, lobster armor. Which is hard to digest and could give you a belly ache, or diarrhea, Which sucks when you are tripping. Not eating the fruit bodies (sporocarps some say) is a great plan. A lot of people have tried strains of P.cubensis, which is easy to cultivate, but thr stronger woodloving species are much tougher and taste terrible. Like eating cat-piss soaked twigs. You gotta really want it, to choke down a few grams of those

 

[Mr Gonzo]

Jumping the extraction comment event though it is very interesting and concise, it is the comments regarding the negative effects that can be had from eating mushrooms that is of the most interesting. As I mentioned eating mushrooms is the way, to want the pure compound I believe that a synth is the way there.

I have eaten so many shrooms the thought even as write makes me gag and feel nauseous, my way around is dried fruit powdered fine and packed into double zero caps, each holds between a gram to 0.7g. not direct taste of them and the fine powder reacts fast, even this comes with the mushroom taste, being sick all manor of discomfort you don't want tripping balls.

My go to mushrooms are liberty caps notoriously potent, and P.cyans the wavy cap another potent mushroom. Liberty caps can give gastric upset, but wood lovers paralysis is common and can freak the most seasoned psyconought out, but double zero gel caps are the way they look like horse pills but do the job well.

My other past time is taking 7g of cyans with 2 or 3 good tabs, this will blow your head clean off. This something I never advise anyone to try even if you feel strong. Personally this is the very top end of my use, and is something I even prepare for months in advanced. Now days even caps of mushies is too much for me to thing of taking due to the gag response mentioned, Love acid maybe take silly amounts and prolonged usage, but love tripping, if I could have tripping on good acid as my reality I would!

 

[Osmosis Vanderwaal]

Yeah that's insane amounts. I've been rendered unconscious by smaller amounts. It's strange to me that magic mushrooms have a distinctive smell. It's unmistakable. Best I can describe it is a freshly cut field and cat piss. I've sworn off of hallucinogenic drugs for about 12 years now. I don't even collect the mushrooms when I see them anymore. I'm a TI on the Shroomery.org but I rarely go on that site anymore

 

[lilscum90]

Have you ever tried lemon tea? It definitely helps with some of the negative side effects of eating them also kicks in alot faster too

 

[itsead]

I was just found by this page and thank you very much for this guide!

Hm so from my knowledge most/alot of the psilocin should already be converted to psilocybin during the drying process.
Please correct me if I am wrong.

https://www.oregon.gov/oha/PH/PREVENTIONWELLNESS/Documents/Stability%20of%20Psilocybin%20and%20Analogs.pdf

I have a friend working on pure water extraction using different methods (low-cost) ultra-sonic etc. lemon-tek vacuum distillation style.

 

[Osmosis Vanderwaal]

I know that dephosforlating psilocybin into psilocin is very easy and happens inside your stomach when you eat mushrooms and I also know that psilocin is poorly soluble in water but very soluble in alcohol. Extracting mushrooms into water never worked for me. The only method that I found highly efficacious was extracting into the strongest ethanol I could get, and mild heat was also necessary and then concentrating it through evaporation. You could concentrate it down to a powerful elixir but then if I put it in the freezer and crystalized it the crystals didn't survive very long in open air

 

[itsead]

Hey Frit, thanks for your reply. The 90% ethanol should be given a try soon. Do you also modify the PH (I think at around 3.5 the blueing is stopped) and do you use any kind of shaker/mixer/homogenizer? aaand have you tried acetone washing too ?

ps. From my knowledge Psilocin is also highly(!) sensitive to light (and also oxygen). So depending on the processing time maybe infrared-lighted lab and brownglass should be used and even the drying should be done in complete darkness.

 

[SoldadoDeDrogas]

I was always under the impression that this was a bad idea and wasn't very practical because of the delicate nature of the psilocybin/psilocin. My trouble was always wanting to eat a good amount of shrooms but not being able to manage the stomach load well - trying to get into your trip with an upset stomach that feels like its boiling, not much fun. Usually ruined the experience for me.

One of the best pieces of advice I was even given about eating shrooms. It helps to be on an empty stomach of course. In any event, have your mushrooms bone dry, so that the stem snaps like a chicken bone. Grind up your mushrooms into a fine powder using a coffee grinder/blender. Mix your shroom powder into a good portion of orange juice and down that shit. It will take about 30 minutes for the onset to begin, the come up is fast, which is a good thing I think, because coming up is usually the worst and only uncomfortable/anxiety prone period of the experience. No stomach load, good clean trip .. hits you all at once. Haven't had any bad or unpleasant experiences really since, that I can really remember. So, hopefully someone finds that bit of advice as useful as I did.

Take care y'all - happy trippin' o7

 

[Osmosis Vanderwaal]

lemon-tek certainly brings it on faster. It's been said the ascorbic acid de-phosphorlates the psilocybin, so it is already converted to 4-Ho-DMT when it hits your stomach. If you put them in some golden grains or everclear and heat the gently and accasionally and shake it up. In 2 to 5 days, filter out the basidoms and evaporate it doen until you have the doeasage you want , 5 ml /g is a goodspot. That will hit you in like 6 minutes flat. No nausea whatsoever, no bellyache, and You can acieve nearly LSD level visuals

 

[ThaiLabWiazard]

Would the following synthesis work? Starting from 4-OH-Indole. Any thoughts?

1) 4-OH-Indole -> 4-Acetoxyindole
2) 4-Acetoxyindole -> 4-Acetoxytryptamine
3) 4-Hydroxytryptamine -> 4-Hydroxy-DMT ((psylocin)
4) (Optional) 4-Hydroxy-DMT -> Psylocybin. (O-Phosphorylation
via PoCl3)